Anusith Thanapimmetha
Bioprocess Laboratory, Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Thailand \ Thailand and The Center of Excellence for Petroleum, Petrochemicals and Advanced Materials, PERDO, Thailand
Korsuk Vuttibunchon
Bioprocess Laboratory, Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Thailand
Maythee Saisriyoot
Bioprocess Laboratory, Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Thailand \ Thailand and The Center of Excellence for Petroleum, Petrochemicals and Advanced Materials, PERDO, Thailand
Penjit Srinophakun
Bioprocess Laboratory, Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Thailand \ Thailand and The Center of Excellence for Petroleum, Petrochemicals and Advanced Materials, PERDO, Thailand
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http://dx.doi.org/10.3384/ecp11057389Published in: World Renewable Energy Congress - Sweden; 8-13 May; 2011; Linköping; Sweden
Linköping Electronic Conference Proceedings 57:52, p. 389-396
Published: 2011-11-03
ISBN: 978-91-7393-070-3
ISSN: 1650-3686 (print), 1650-3740 (online)
Sweet sorghum (Sorghum bicolor L. Moench) is the potential raw material of ethanol production. As a lignocellulosic material; hydrolysis is needed to transform it to sugar. Usually; this sugar was used as substrate for ethanol production by fermentation. In this study; the composition of sweet sorghum bagasse was analyzed. The compositions of the sweet sorghum bagasse before hydrolysis are 58.23% cellulose; 25.42% hemicellulose and 14.95% lignin. It was then cut; dried and pretreated at 121 oC; 25 min by sodium hydroxide. Then the experimental design is performed to design the experimental runs. Box-Behnken was used to design the experiment of chemical hydrolysis. The factors of sulfuric acid concentration (15-55 %w/w); solid to liquid ratio (1:10-1:30 w:w) and reaction time (40-120 minutes) affecting reducing sugar production were optimized for the chemical hydrolysis. At the optimum condition; the maximum reducing sugar was equal to 33.49% (g/g dry substrate). On the other hand; microbial hydrolysis with Trichoderma harzianum gave the maximum reducing sugar of 10.34 % (g/g dry substrate) at optimum condition. Then type of reducing sugar was analyzed using High Performance Liquid Chromatography (HPLC). It is obvious that glucose is the dominated reducing sugar. Finally; the reducing sugar (glucose; xylose and arabinose) from chemical and microbial hydrolysis is usually fermented with Saccharomyces cerevisiae to produce ethanol.
