Konferensartikel

A Comparative Study of Immobilized-Whole Cell and Commercial Lipase as a Biocatalyst for Biodiesel Production from Soybean Oil

S. N. Hashemizadeh
School of Chemical Engineering, College of Engineering, University of Tehran, Iran \ Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

O. Tavakoli
School of Chemical Engineering, College of Engineering, University of Tehran, Iran

F. Tabandeh
Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

A. A. Karkhane
Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Z. Forghanipour
School of Chemical Engineering, College of Engineering, University of Tehran, Iran \ Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Ladda ner artikelhttp://dx.doi.org/10.3384/ecp11057311

Ingår i: World Renewable Energy Congress - Sweden; 8-13 May; 2011; Linköping; Sweden

Linköping Electronic Conference Proceedings 57:42, s. 311-318

Visa mer +

Publicerad: 2011-11-03

ISBN: 978-91-7393-070-3

ISSN: 1650-3686 (tryckt), 1650-3740 (online)

Abstract

Recently; there has been considerable attention in the direct use of intracellular lipase as a whole-cell biocatalyst (indirect immobilization of the enzyme) for biodiesel production since immobilization can be carried out spontaneously during the process of cell cultivation. In this research the ability of Rhizopus oryzae (ATCC 9374) whole-cell biocatalyst that was immobilized within biomass support particles (BSPs) was investigated and compared with Novozym 435 (most effective extracellular immobilized lipase) for methanolysis of soybean oil in solvent free system. The maximum methyl esters content in the reaction mixture reaches 84 wt% using R.oryzae whole-cell biocatalyst in optimum condition (6mm×6mm×3mm BSPs size; olive oil as carbon sources in basel medium; emulsification using ultrasonicated of reaction mixtures; 15 wt% water content and 7 wt% immobilized BSPs; addition of methanol at 0; 4 and 18 h) and at reaction time of 48 h which is remarkably comparable with yield of biodiesel at 90 wt% obtained with Novozym 435. Both the lipases can be used for repeated batches cycles. These findings indicate that; given the simplicity of the lipase production process and the long-term stability of lipase activity; the use of whole-cell biocatalysts immobilized within BSPs and treated with glutaraldehyde solution suggest a favorable means of biodiesel fuel production for industrial application.

Nyckelord

Biodiesel; Whole-Cell Biocatalyst; Rhizopus oryzae; Novozym 435

Referenser

[1] M. S. Antczak; A. Kubiak; T. Antczak; S. Bielecki; Enzymatic biodiesel synthesis – Key factors affecting efficiency of the process; Renewable Energy 34; 2009; 1185–1194. doi: 10.1016/j.renene.2008.11.013.

[2] H. Fukuda; A. Kondo; H. Noda; Biodiesel fuel production by transesterification of oils; J. Biosci. Bioeng. 92; 5; 2001; 405–416.

[3] P. S. Bisen; B. S. Sanodiya; G. S. Thakur; R. K. Baghel; G. B. K. S. Prasad; Biodiesel production with special emphasis on lipase-catalyzed transesterification; Biotechnology Letters 32; 2010; 8; 1019-1030.

[4] H. Fukuda; S. Hama; S. Tamalampudi and H. Noda; Whole-cell biocatalysts for biodiesel fuel production; Trend in biotechnology 26; 2008; 12; 668-673.

[5] A. Robles-Medina; P.A. González-Moreno; L. Esteban-Cerdán; E. Molina-Grima; Biocatalysis: Towards ever greener biodiesel production; Biotechnology Advances 27; 2009; 398–408. doi: 10.1016/j.biotechadv.2008.10.008.

[6] Y. Shimada; Y.Watanabe; T. Samukawa; A. Sugihara; H. Noda; H. Fukuda; Y. Tominaga; Conversion of vegetable oil to biodiesel using immobilized Candida antarctica lipase; J. Am. Oil Chem. Soc. 76; 1999; 789–793. doi: 10.1007/s11746-999-0067-6.

[7] Y. Xu; W. Du; D. Liu; J. Zeng; A novel enzymatic route for biodiesel production from renewable oils in a solvent-free medium; Biotechnology Letters 25; 2003; 1239–1241. doi: 10.1023/A:1025065209983.

[8] W. Du; Y. Xu; D. Liu; J. Zeng; Comparative study on lipase-catalyzed transformation of soybean oil for biodiesel production with different acyl acceptors; J. Mol. Catal. B: Enzym. 30; 2004; 125–129. doi: 10.1016/j.molcatb.2004.04.004.

[9] S. V. Ranganathan; S. L. Narasimhan; K. Muthukumar; An overview of enzymatic production of biodiesel”; Bioresource Technology 99; 2008; 3975–3981. doi: 10.1016/j.biortech.2007.04.060.

[10] Ting Sun; Wei Du and Dehua Liu; Prospective and impacts of whole cell mediated alcoholysis of renewable oils for biodiesel production; Biofuels; Bioprod. Bioref. 3; 2009; 633–639. doi: 10.1002/bbb.180.

[11] K. Ban; S. Hama; K. Nishizuka; M. Kaieda; T. Matsumoto; A. Kondo ; H. Noda; H. Fukuda; Repeated use of whole-cell biocatalysts immobilized within biomass support particles for biodiesel fuel production; Journal of Molecular Catalysis B: Enzymatic 17; 2002; 157–165. doi: 10.1016/S1381-1177(02)00023-1.

[12] K. Ban; M. Kaieda; T. Matsumoto; A. Kondo; H. Fukudac; Whole cell biocatalyst for biodiesel fuel production utilizing Rhizopus oryzae cells immobilized within biomass support particles; Biochemical Engineering Journal 8; 2001; 39–43. doi: 10.1016/S1369-703X(00)00133-9.

[13] S. Tamalampudi; M. R. Talukder; S. Hama; T. Numata; A. Kondo; H. Fukuda; Enzymatic production of biodiesel from Jatropha oil: A comparative study of immobilized-whole cell and commercial lipases as a biocatalyst; Biochemical Engineering Journal 39; 2008; 185–189. doi: 10.1016/j.bej.2007.09.002.

[14] Y. Shimada; Y. Watanabe; A. Sugihara; Y. Tominaga; Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing; J. Mol. Catal. B: Enzym. 76; 2002; 133–142. doi: 10.1016/S1381-1177(02)00020-6.

[15] S. Hama; H. Yamaji; T. Fukumizu; T. Numata; S. Tamalampudi ; A. Kondo; H. Noda; H. Fukuda; Biodiesel-fuel production in a packed-bed reactor using lipase-producing Rhizopus oryzae cells immobilized within biomass support particles; Biochemical Engineering Journal 34; 2007; 273–278. doi: 10.1016/j.bej.2006.12.013.

[16] R.H. Valivety; G.A. Johnston; C.J. Suckling; P.J. Halling; Solvent effects on biocatalysis in organic systems: equilibrium position and rates of lipase-catalysed esterification; Biotechnol. Bioeng. 38; 1991; 1137–1143. doi: 10.1002/bit.260381004.

Citeringar i Crossref