Konferensartikel

Development of Laccase and Manganese Peroxidase Biocathodes for Microbial Fuel Cell Applications

Sahar Bakhshian
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran

Hamid-Reza Kariminia
Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran

Ladda ner artikelhttp://dx.doi.org/10.3384/ecp110571197

Ingår i: World Renewable Energy Congress - Sweden; 8-13 May; 2011; Linköping; Sweden

Linköping Electronic Conference Proceedings 57:9, s. 1197-1204

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Publicerad: 2011-11-03

ISBN: 978-91-7393-070-3

ISSN: 1650-3686 (tryckt), 1650-3740 (online)

Abstract

In this study; we ivestigated how microbial fuel cell (MFC) performance can be affected by laccase and manganese peroxidase (MnP) enzymes as catalysts in the cathode compartment. Commercial laccase was immobilized by crosslinking on chitosan using glutaraldehyde. Immobilized enzyme was settled on graphite electrode previously covered with polymerized methylene blue. Application of this enzymatic electrode was investigated in the cathode chamber of a MFC. Output power density of the MFC in the mentioned situation was 100% higher than that for the graphite electrode. The MnP was first; produced from a white rot fungus isolate and was immobilized on the graphite electrode via adsorption. This modified electrode with MnP was utilized as cathode. The fuel cell with MnP modified graphite electrode and H2O2 as oxidizer yielded the maximum power density of 46 mW/m2 at the current density of 109 mA/m2. This augmentation of MFC performance was due to a higher cathode electrode potential with H2O2 rather than oxygen. The most important function of MnP was to catalyze the reduction of H2O2 and hence diminished activation overpotential loss of the cathode.

Nyckelord

Biocathode; Laccase; Manganese peroxidase; Microbial fuel cell

Referenser

[1] B.E. Logan; B. Hamelers; R. Rozendal; U. Schröder; J. Keller; S. Freguia; P. Aelterman; W. Verstraete and K. Rabaey; Microbial fuel cells: methodology and technology; Environmental Science and Technology; 2006; pp. 307-314.

[2] Z.-D. Liu; H.-R. Li; Effects of bio- and abio-factors on electricity production in a mediatorless microbial fuel cell; Biochemical Engineering Journal; 2007; pp. 209-214. doi: 10.1016/j.bej.2007.02.021.

[3] R.D. Lovely; Microbial fuel cells: novel microbial physiologies and engineering approaches; Current Opinion in Biotechnology; 2006; pp. 327-332. doi: 10.1016/j.copbio.2006.04.006.

[4] G. Tayhas; R. Palmore; H.H. Kim; Electro-enzymatic reduction of dioxygen to water in the cathode compartment of a biofuel cell; Journal of Electroanalytical Chemistry; 1999; pp. 110-117.

[5] O. Lefebvre; W.K. Ooi; Z. Tang; Md. Abdullah-Al-Mamun; D.H.C. Chua; H.Y. Ng; Optimization of a Pt-free cathode suitable for practical applications of microbial fuel cells; Bioresource Technology; 2009; pp. 4907-4910. doi: 10.1016/j.biortech.2009.04.061.

[6] M. Smolander; H. Boer; M. Valkiainen; R. Roozemana; M. Bergelin; J. E. Eriksson; X. C. Zhang; A. Koivula; L. Viikari; Development of a printable laccase-based biocathode for fuel cell applications; Enzyme and Microbial Technology; 2008; pp. 93-102. doi: 10.1016/j.enzmictec.2007.11.019.

[7] R.A. Bullen; T.C. Arnot; J.B. Lakeman; F.C. Walsh; Biofuel cells and their development; Biosensors and Bioelectronics; 2006; pp. 2015-2045. doi: 10.1016/j.bios.2006.01.030.

[8] N. Duran; M. Rosa; A.D. Annibale; L. Gianfreda; Application of laccases and tyrosinases (phenoloxidases) immobilized on different supports: a review; Enzyme and Microbial Technology; 2002; pp. 907-931. doi: 10.1016/S0141-0229(02)00214-4.

[9] C. Vaz-Dominguez; S. Campuzano; O. Rudiger; M. Pita; M. Gorbacheva; S. Shleev; V. M. Fernandez; A.L.D. Lacey; Laccase electrode for direct electrocatalytic reduction of O2 to H2O with high-operational stability and resistance to chloride inhibition; Biosensors and Bioelectronics; 2008; pp. 531-537. doi: 10.1016/j.bios.2008.05.002.

[10] H.-R. Kariminiaae-Hamedaani; A. Sakurai; M. Sakakibara; Decolorization of synthetic dyes by a new manganese peroxidase-producing white rot fungus. Dyes and Pigments; 2007; pp. 157-162. doi: 10.1016/j.dyepig.2005.08.010.

[11] M. Hofrichter; Review: lignin conversion by manganese peroxidase (MnP); Enzyme and Microbial Technology; 2003; pp. 454-466.

[12] J. Zhang; Z. Xu; H. Chen; Y. Zong; Removal of 2;4-dichlorophenol by chitosan-immobilized laccase from Coriolus versicolor; Biochemical Engineering Journal; 2009; pp. 54-59. doi: 10.1016/j.bej.2009.02.005.

[13] A.A. Karyakin; E.E. Karyakina; H.-L. Schmidt; Electropolymerized azines: a new group of electroactive polymers; Electroanalysis; 1999; pp. 149-155. doi: 10.1002/(SICI)1521-4109(199903)11:3<149::AID-ELAN149>3.0.CO;2-G.

[14] X.Q. Yang; X.X. Zhao; C.Y. Liu; Y. Zheng; S.J. Qian; Decolorization of azo; triphenylmethane and anthraquinone dyes by a newly isolated Trametes sp. SQ01 and its laccase; Process Biochemistry; 2009; pp. 1185-1189. doi: 10.1016/j.procbio.2009.06.015.

[15] H. Wariishi; K. Valli; MH. Gold; Manganese (II) oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium; Journal of Biological Chemistry; 1992; pp. 23688-23695.

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